Class: Bioroebe::RestrictionEnzyme
- Inherits:
-
CommandlineApplication
- Object
- Base
- CommandlineApplication
- Bioroebe::RestrictionEnzyme
- Defined in:
- lib/bioroebe/enzymes/restriction_enzyme.rb
Overview
Bioroebe::RestrictionEnzyme
Constant Summary collapse
- HASH_ALL_RESTRICTION_ENYMES =
#
HASH_ALL_RESTRICTION_ENYMES
#
Bioroebe.restriction_enzymes(:hash)
Constants inherited from CommandlineApplication
CommandlineApplication::OLD_VERBOSE_VALUE
Constants included from ColoursForBase
ColoursForBase::ARRAY_HTML_COLOURS_IN_USE
Constants inherited from Base
Class Method Summary collapse
-
.[](i = ARGV) ⇒ Object
# === Bioroebe::RestrictionEnzyme[] ========================================================================= #.
Instance Method Summary collapse
-
#can_cut_this_sequence?(i) ⇒ Boolean
# === can_cut_this_sequence? ========================================================================= #.
-
#cuts_at? ⇒ Boolean
(also: #recognizes_which_sequence?)
# === cuts_at?.
-
#do_cut_the_sequence(this_sequence = 'GGGGATTCATGCCAAGGGGAATTCGATTCATGCCAAAAAAAAATGAATTCTTTTTAAAAAACCCCCGGGGGGTTT') ⇒ Object
(also: #cut_the_sequence)
# === do_cut_the_sequence.
-
#initialize(commandline_arguments = nil, run_already = true) ⇒ RestrictionEnzyme
constructor
# === initialize ========================================================================= #.
-
#reset ⇒ Object
# === reset (reset tag) ========================================================================= #.
-
#return_original_sequence ⇒ Object
# === return_original_sequence.
-
#run ⇒ Object
# === run (run tag) ========================================================================= #.
-
#set_cuts_at(i = @dataset.first) ⇒ Object
# === set_cuts_at ========================================================================= #.
-
#set_name_of_the_restriction_enzyme(i) ⇒ Object
# === set_name_of_the_restriction_enzyme ========================================================================= #.
-
#use_this_restriction_enzyme=(i = :EcoRI) ⇒ Object
# === use_this_restriction_enzyme= ========================================================================= #.
Methods inherited from CommandlineApplication
#all_aminoacids?, #append_what_into, #at_home?, #be_silent, #be_verbose?, #cat, #ccliner, #change_directory, #cliner, #codon_table_dataset?, #codon_to_aminoacid, #codons_for?, #colourize_this_dna_sequence, #complement, #cp, #disable_warnings, #download_dir?, #editor?, #enable_warnings, #ensure_that_the_base_directories_exist, #esystem, #extract, #is_this_a_start_codon?, #is_this_a_stop_codon?, #leading_five_prime, #load_bioroebe_yaml_file, #log_directory?, #one_letter_to_long_name, #one_to_three, #only_numbers?, #open_in_browser, #opnerev, #opnn, #pad_with_double_quotes, #pad_with_single_quotes, #partner_nucleotide, #remove_numbers, #remove_trailing_ansii_escape_code, #return_all_possible_start_codons, #return_array_of_one_letter_aminoacids, #return_cheerful_person, #return_chunked_display, #return_ubiquitin_sequence, #runmode?, #set_be_verbose, #set_runmode, #start_codon?, #stop_codons?, #strict_filter_away_invalid_aminoacids, #taxonomy_download_directory?, #three_to_one, #to_rna, #trailing_three_prime, #use_opn?, #verbose_truth, #was_or_were, #without_extname, #write_what_into
Methods included from BaseModule
#absolute_path, #default_file_read, #file_readlines
Methods included from CommandlineArguments
#commandline_arguments?, #commandline_arguments_that_are_files?, #e, #first?, #first_non_hyphen_argument?, #remove_hyphens_from_the_commandline_arguments, #return_commandline_arguments_as_string, #return_commandline_arguments_that_are_not_files, #return_entries_without_two_leading_hyphens, #select_commandline_arguments, #select_entries_starting_with_two_hyphens, #set_commandline_arguments
Methods included from ColoursForBase
#colourize_this_aminoacid_sequence_for_the_commandline, #colourize_this_nucleotide_sequence, #disable_colours, #ecomment, #efancy, #egold, #enable_colours, #eorange, #eparse, #erev, #red, #remove_trailing_escape_part, #return_colour_for_nucleotides, #rev, #sdir, #set_will_we_use_colours, #sfancy, #sfile, #simp, #swarn, #use_colours?, #use_colours_within_the_bioroebe_namespace?
Methods inherited from Base
#append_what_into, #can_base_pair?, #convert_global_env, #delete_file, #directory_to_the_codon_tables?, #is_on_roebe?, #is_palindrome?, #main_encoding?, #mkdir, #move_file, #mv, #no_file_exists_at, #no_newlines, #project_yaml_directory?, #rds, #register_sigint, #return_pwd, #return_the_first_line_of_this_file, #word_wrap, #write_what_into
Methods included from InternalHashModule
#internal_hash?, #reset_the_internal_hash
Methods included from InferTheNamespaceModule
#infer_the_namespace, #namespace?
Constructor Details
#initialize(commandline_arguments = nil, run_already = true) ⇒ RestrictionEnzyme
#
initialize
#
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# File 'lib/bioroebe/enzymes/restriction_enzyme.rb', line 46 def initialize( commandline_arguments = nil, run_already = true ) reset set_commandline_arguments( commandline_arguments ) run if run_already end |
Class Method Details
.[](i = ARGV) ⇒ Object
#
Bioroebe::RestrictionEnzyme[]
#
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# File 'lib/bioroebe/enzymes/restriction_enzyme.rb', line 155 def self.[](i = ARGV) new(i) end |
Instance Method Details
#can_cut_this_sequence?(i) ⇒ Boolean
#
can_cut_this_sequence?
#
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# File 'lib/bioroebe/enzymes/restriction_enzyme.rb', line 115 def can_cut_this_sequence?(i) i.include? @cuts_at end |
#cuts_at? ⇒ Boolean Also known as: recognizes_which_sequence?
#
cuts_at?
This method will return at which sequence to cut.
#
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# File 'lib/bioroebe/enzymes/restriction_enzyme.rb', line 88 def cuts_at? @cuts_at end |
#do_cut_the_sequence(this_sequence = 'GGGGATTCATGCCAAGGGGAATTCGATTCATGCCAAAAAAAAATGAATTCTTTTTAAAAAACCCCCGGGGGGTTT') ⇒ Object Also known as: cut_the_sequence
#
do_cut_the_sequence
This method can take a sequence and cut it. Note that the individual fragments return are not the final digestion - we still have to find out where the restriction enzyme really cuts.
#
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# File 'lib/bioroebe/enzymes/restriction_enzyme.rb', line 135 def do_cut_the_sequence( this_sequence = 'GGGGATTCATGCCAAGGGGAATTCGATTCATGCCAAAAAAAAATGAATTCTTTTTAAAAAACCCCCGGGGGGTTT' ) if can_cut_this_sequence?(this_sequence) splitted = this_sequence.split(/#{@cuts_at}/) return splitted else [this_sequence] end end |
#reset ⇒ Object
#
reset (reset tag)
#
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# File 'lib/bioroebe/enzymes/restriction_enzyme.rb', line 60 def reset super() # ======================================================================= # # === @type # ======================================================================= # @type = nil # ======================================================================= # # === @cuts_at # # Denote where the restriction enzyme will cut at. # ======================================================================= # @cuts_at = '' end |
#return_original_sequence ⇒ Object
#
return_original_sequence
This method will return the original sequence as-is.
#
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# File 'lib/bioroebe/enzymes/restriction_enzyme.rb', line 124 def return_original_sequence HASH_ALL_RESTRICTION_ENYMES[@name_of_the_restriction_enzyme] end |
#run ⇒ Object
#
run (run tag)
#
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# File 'lib/bioroebe/enzymes/restriction_enzyme.rb', line 149 def run end |
#set_cuts_at(i = @dataset.first) ⇒ Object
#
set_cuts_at
#
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# File 'lib/bioroebe/enzymes/restriction_enzyme.rb', line 77 def set_cuts_at( i = @dataset.first ) @cuts_at = i end |
#set_name_of_the_restriction_enzyme(i) ⇒ Object
#
set_name_of_the_restriction_enzyme
#
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# File 'lib/bioroebe/enzymes/restriction_enzyme.rb', line 95 def set_name_of_the_restriction_enzyme(i) @name_of_the_restriction_enzyme = i.to_s end |
#use_this_restriction_enzyme=(i = :EcoRI) ⇒ Object
#
use_this_restriction_enzyme=
#
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# File 'lib/bioroebe/enzymes/restriction_enzyme.rb', line 102 def use_this_restriction_enzyme=(i = :EcoRI) set_name_of_the_restriction_enzyme(i) @dataset = HASH_ALL_RESTRICTION_ENYMES[i.to_s] if @dataset.include? ' ' set_cuts_at(@dataset.split(' ').first) else set_cuts_at(@dataset.first) end end |