Class: Bioroebe::AlignOpenReadingFrames
Overview
Bioroebe::AlignOpenReadingFrames
Constant Summary
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- START_CODON =
::Bioroebe.start_codon?
- SHOW_N_NUCLEOTIDES =
true
CommandlineApplication::OLD_VERBOSE_VALUE
ColoursForBase::ARRAY_HTML_COLOURS_IN_USE
Constants inherited
from Base
Base::NAMESPACE
Class Method Summary
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-
.[](i = '') ⇒ Object
# === Bioroebe::AlignOpenReadingFrames[] ========================================================================= #.
Instance Method Summary
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#all_aminoacids?, #append_what_into, #at_home?, #be_silent, #be_verbose?, #cat, #ccliner, #change_directory, #cliner, #codon_table_dataset?, #codon_to_aminoacid, #codons_for?, #colourize_this_dna_sequence, #complement, #cp, #disable_warnings, #download_dir?, #editor?, #enable_warnings, #ensure_that_the_base_directories_exist, #esystem, #extract, #is_this_a_start_codon?, #is_this_a_stop_codon?, #leading_five_prime, #load_bioroebe_yaml_file, #log_directory?, #one_letter_to_long_name, #one_to_three, #only_numbers?, #open_in_browser, #opnerev, #opnn, #pad_with_double_quotes, #pad_with_single_quotes, #partner_nucleotide, #remove_numbers, #remove_trailing_ansii_escape_code, #return_all_possible_start_codons, #return_array_of_one_letter_aminoacids, #return_cheerful_person, #return_chunked_display, #return_ubiquitin_sequence, #runmode?, #set_be_verbose, #set_runmode, #stop_codons?, #strict_filter_away_invalid_aminoacids, #taxonomy_download_directory?, #three_to_one, #to_rna, #trailing_three_prime, #use_opn?, #verbose_truth, #was_or_were, #without_extname, #write_what_into
Methods included from BaseModule
#absolute_path, #default_file_read, #file_readlines
#commandline_arguments?, #commandline_arguments_that_are_files?, #e, #first?, #first_non_hyphen_argument?, #remove_hyphens_from_the_commandline_arguments, #return_commandline_arguments_as_string, #return_commandline_arguments_that_are_not_files, #return_entries_without_two_leading_hyphens, #select_commandline_arguments, #select_entries_starting_with_two_hyphens, #set_commandline_arguments
#colourize_this_aminoacid_sequence_for_the_commandline, #colourize_this_nucleotide_sequence, #disable_colours, #ecomment, #efancy, #egold, #enable_colours, #eorange, #eparse, #erev, #red, #remove_trailing_escape_part, #return_colour_for_nucleotides, #rev, #sdir, #set_will_we_use_colours, #sfancy, #sfile, #simp, #swarn, #use_colours?, #use_colours_within_the_bioroebe_namespace?
Methods inherited from Base
#append_what_into, #can_base_pair?, #convert_global_env, #delete_file, #directory_to_the_codon_tables?, #is_on_roebe?, #is_palindrome?, #main_encoding?, #mkdir, #move_file, #mv, #no_file_exists_at, #no_newlines, #project_yaml_directory?, #rds, #register_sigint, #return_pwd, #return_the_first_line_of_this_file, #word_wrap, #write_what_into
#internal_hash?, #reset_the_internal_hash
#infer_the_namespace, #namespace?
Constructor Details
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# File 'lib/bioroebe/utility_scripts/align_open_reading_frames.rb', line 52
def initialize(
i = nil,
run_already = true
)
reset
set_input(i)
run if run_already
end
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Class Method Details
.[](i = '') ⇒ Object
#
Bioroebe::AlignOpenReadingFrames[]
#
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# File 'lib/bioroebe/utility_scripts/align_open_reading_frames.rb', line 175
def self.[](i = '')
new(i)
end
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Instance Method Details
#analyze_main_sequence ⇒ Object
#
analyze_main_sequence
#
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# File 'lib/bioroebe/utility_scripts/align_open_reading_frames.rb', line 134
def analyze_main_sequence
array = ::Bioroebe.scan_this_input_for_startcodons(input?, return_regex_with_startcodons)
if array.empty?
report_that_no_ORF_has_been_found
else
report_aligned_sequences_using_this_array(
array.map {|inner_array| inner_array.first }
)
end
end
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#determine_n_nucleotides_in(i) ⇒ Object
#
determine_n_nucleotides_in
#
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# File 'lib/bioroebe/utility_scripts/align_open_reading_frames.rb', line 125
def determine_n_nucleotides_in(i)
result = "(#{i.size.to_s} nucleotides)"
result = slategrey(result) if use_colours?
return result
end
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# File 'lib/bioroebe/utility_scripts/align_open_reading_frames.rb', line 81
def input?
@input
end
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#report_aligned_sequences_using_this_array(array) ⇒ Object
#
report_aligned_sequences_using_this_array
This method will report all sequences in an aligned manner.
#
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# File 'lib/bioroebe/utility_scripts/align_open_reading_frames.rb', line 97
def report_aligned_sequences_using_this_array(array)
_ = input? e
array.each_with_index {|start_position, index|
index += 1
sequence = _[(start_position - 1) .. -1]
if use_colours?
sequence.gsub!(/#{start_codon?}/,
::Colours.orange(start_codon?)+rev)
end
this_orf = 'ORF #'+index.to_s
if this_orf.include? 'ORF #1'
this_orf << "#{rev} (#{steelblue('longest ORF')})"
end
e simp(this_orf).rjust(5)+rev
e
result = " #{sequence}".dup
if SHOW_N_NUCLEOTIDES
result << " #{determine_n_nucleotides_in(sequence)}"
end
e result
e
}
end
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#report_that_no_ORF_has_been_found ⇒ Object
#
report_that_no_ORF_has_been_found
#
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# File 'lib/bioroebe/utility_scripts/align_open_reading_frames.rb', line 152
def report_that_no_ORF_has_been_found
this_length = input?.size
opnerev "No valid stop-codon has been found for the given input"
opnerev "sequence of length #{sfancy(this_length.to_s)}."
end
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#reset ⇒ Object
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# File 'lib/bioroebe/utility_scripts/align_open_reading_frames.rb', line 64
def reset
super()
infer_the_namespace
end
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#return_regex_with_startcodons ⇒ Object
#
return_regex_with_startcodons
#
#run ⇒ Object
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# File 'lib/bioroebe/utility_scripts/align_open_reading_frames.rb', line 168
def run
analyze_main_sequence
end
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# File 'lib/bioroebe/utility_scripts/align_open_reading_frames.rb', line 72
def set_input(i = '')
i = i.first if i.is_a? Array
i = i.to_s.dup
@input = i
end
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#start_codon? ⇒ Boolean
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# File 'lib/bioroebe/utility_scripts/align_open_reading_frames.rb', line 88
def start_codon?
START_CODON
end
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