Class: Bio::UniProtKB

Inherits:
EMBLDB show all
Includes:
EMBLDB::Common
Defined in:
lib/bio/db/embl/uniprotkb.rb

Overview

Description

Parser class for UniProtKB/SwissProt and TrEMBL database entry.

See the UniProtKB document files and manuals.

Examples

str = File.read("p53_human.swiss")
obj = Bio::UniProtKB.new(str)
obj.entry_id #=> "P53_HUMAN"

References

Direct Known Subclasses

TrEMBL

Constant Summary collapse

@@entry_regrexp = 
/[A-Z0-9]{1,4}_[A-Z0-9]{1,5}/
@@data_class = 
["STANDARD", "PRELIMINARY"]
@@ac_regrexp =

Bio::EMBLDB::Common#ac -> ary

#accessions  -> ary
#accession  -> String (accessions.first)

/[OPQ][0-9][A-Z0-9]{3}[0-9]/
@@cc_topics = 
['PHARMACEUTICAL',
'BIOTECHNOLOGY',
'TOXIC DOSE', 
'ALLERGEN',   
'RNA EDITING',
'POLYMORPHISM',
'BIOPHYSICOCHEMICAL PROPERTIES',
'MASS SPECTROMETRY',
'WEB RESOURCE', 
'ENZYME REGULATION',
'DISEASE',
'INTERACTION',
'DEVELOPMENTAL STAGE',
'INDUCTION',
'CAUTION',
'ALTERNATIVE PRODUCTS',
'DOMAIN',
'PTM',
'MISCELLANEOUS',
'TISSUE SPECIFICITY',
'COFACTOR',
'PATHWAY',
'SUBUNIT',
'CATALYTIC ACTIVITY',
'SUBCELLULAR LOCATION',
'FUNCTION',
'SIMILARITY']
@@dr_database_identifier =

returns databases cross-references in the DR lines.

  • Bio::UniProtKB#dr -> Hash w/in Array

DR Line; defabases cross-reference (>=0)

  DR  database_identifier; primary_identifier; secondary_identifier.
a cross_ref pre one line

['EMBL','CARBBANK','DICTYDB','ECO2DBASE',
'ECOGENE',
'FLYBASE','GCRDB','HIV','HSC-2DPAGE','HSSP','INTERPRO','MAIZEDB',
'MAIZE-2DPAGE','MENDEL','MGD''MIM','PDB','PFAM','PIR','PRINTS',
'PROSITE','REBASE','AARHUS/GHENT-2DPAGE','SGD','STYGENE','SUBTILIST',
'SWISS-2DPAGE','TIGR','TRANSFAC','TUBERCULIST','WORMPEP','YEPD','ZFIN']

Constants included from EMBLDB::Common

EMBLDB::Common::DELIMITER, EMBLDB::Common::RS, EMBLDB::Common::TAGSIZE

Instance Method Summary collapse

Methods included from EMBLDB::Common

#ac, #accession, #de, #initialize, #kw, #oc, #og

Methods inherited from EMBLDB

#initialize

Methods inherited from DB

#exists?, #fetch, #get, open, #tags

Instance Method Details

#cc(topic = nil) ⇒ Object

returns contents in the CC lines.

  • Bio::UniProtKB#cc -> Hash

returns an object of contents in the TOPIC.

  • Bio::UniProtKB#cc(TOPIC) -> Array w/in Hash, Hash

returns contents of the “ALTERNATIVE PRODUCTS”.

  • Bio::UniProtKB#cc(‘ALTERNATIVE PRODUCTS’) -> Hash

    {'Event' => str, 
 'Named isoforms' => int,  
 'Comment' => str,
 'Variants'=>[{'Name' => str, 'Synonyms' => str, 'IsoId' => str, 'Sequence' => []}]}

CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=15;
...
CC         placentae isoforms. All tissues differentially splice exon 13;
CC       Name=A; Synonyms=no del;
CC         IsoId=P15529-1; Sequence=Displayed;

returns contents of the “DATABASE”.

  • Bio::UniProtKB#cc(‘DATABASE’) -> Array

    [{'NAME'=>str,'NOTE'=>str, 'WWW'=>URI,'FTP'=>URI}, ...]

CC   -!- DATABASE: NAME=Text[; NOTE=Text][; WWW="Address"][; FTP="Address"].

returns contents of the “MASS SPECTROMETRY”.

  • Bio::UniProtKB#cc(‘MASS SPECTROMETRY’) -> Array

    [{'MW"=>float,'MW_ERR'=>float, 'METHOD'=>str,'RANGE'=>str}, ...]

CC   -!- MASS SPECTROMETRY: MW=XXX[; MW_ERR=XX][; METHOD=XX][;RANGE=XX-XX].

CC lines (>=0, optional)

CC   -!- TISSUE SPECIFICITY: HIGHEST LEVELS FOUND IN TESTIS. ALSO PRESENT
CC       IN LIVER, KIDNEY, LUNG AND BRAIN.

CC   -!- TOPIC: FIRST LINE OF A COMMENT BLOCK;
CC       SECOND AND SUBSEQUENT LINES OF A COMMENT BLOCK.

See also www.expasy.org/sprot/userman.html#CC_line



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# File 'lib/bio/db/embl/uniprotkb.rb', line 774

def cc(topic = nil)
  unless @data['CC']
    cc  = Hash.new
    comment_border= '-' * (77 - 4 + 1)
    dlm = /-!- /

    # 12KD_MYCSM has no CC lines.
    return cc if get('CC').size == 0
    
    cc_raw = fetch('CC')

    # Removing the copyright statement.
    cc_raw.sub!(/ *---.+---/m, '')

    # Not any CC Lines without the copyright statement.
    return cc if cc_raw == ''

    begin
      cc_raw, copyright = cc_raw.split(/#{comment_border}/)[0]
      _ = copyright #dummy for suppress "assigned but unused variable"
      cc_raw = cc_raw.sub(dlm,'')
      cc_raw.split(dlm).each do |tmp|
        tmp = tmp.strip

        if /(^[A-Z ]+[A-Z]): (.+)/ =~ tmp
          key  = $1
          body = $2
          body.gsub!(/- (?!AND)/,'-')
          body.strip!
          unless cc[key]
            cc[key] = [body]
          else
            cc[key].push(body)
          end
        else
          raise ["Error: [#{entry_id}]: CC Lines", '"', tmp, '"',
                 '', get('CC'),''].join("\n")
        end
      end
    rescue NameError
      if fetch('CC') == ''
        return {}
      else
        raise ["Error: Invalid CC Lines: [#{entry_id}]: ",
               "\n'#{self.get('CC')}'\n", "(#{$!})"].join
      end
    rescue NoMethodError
    end
    
    @data['CC'] = cc
  end


  case topic
  when 'ALLERGEN'
    return @data['CC'][topic]
  when 'ALTERNATIVE PRODUCTS'
    return cc_alternative_products(@data['CC'][topic])
  when 'BIOPHYSICOCHEMICAL PROPERTIES'
    return cc_biophysiochemical_properties(@data['CC'][topic])
  when 'BIOTECHNOLOGY'
    return @data['CC'][topic]
  when 'CATALITIC ACTIVITY'
    return cc_catalytic_activity(@data['CC'][topic])
  when 'CAUTION'
    return cc_caution(@data['CC'][topic])
  when 'COFACTOR'
    return @data['CC'][topic]
  when 'DEVELOPMENTAL STAGE'
    return @data['CC'][topic].join('')
  when 'DISEASE'
    return @data['CC'][topic].join('')
  when 'DOMAIN'
    return @data['CC'][topic]
  when 'ENZYME REGULATION'
    return @data['CC'][topic].join('')
  when 'FUNCTION'
    return @data['CC'][topic].join('')
  when 'INDUCTION'
    return @data['CC'][topic].join('')
  when 'INTERACTION'
    return cc_interaction(@data['CC'][topic])
  when 'MASS SPECTROMETRY'
    return cc_mass_spectrometry(@data['CC'][topic])
  when 'MISCELLANEOUS'
    return @data['CC'][topic]
  when 'PATHWAY'
    return cc_pathway(@data['CC'][topic])
  when 'PHARMACEUTICAL'
    return @data['CC'][topic]
  when 'POLYMORPHISM'
    return @data['CC'][topic]
  when 'PTM'
    return @data['CC'][topic]
  when 'RNA EDITING'
    return cc_rna_editing(@data['CC'][topic])
  when 'SIMILARITY'
    return @data['CC'][topic]
  when 'SUBCELLULAR LOCATION'
    return cc_subcellular_location(@data['CC'][topic])
  when 'SUBUNIT'
    return @data['CC'][topic]
  when 'TISSUE SPECIFICITY'
    return @data['CC'][topic]
  when 'TOXIC DOSE'
    return @data['CC'][topic]
  when 'WEB RESOURCE'
    return cc_web_resource(@data['CC'][topic])
  when 'DATABASE'
    # DATABASE: NAME=Text[; NOTE=Text][; WWW="Address"][; FTP="Address"].
    tmp = Array.new
    db = @data['CC']['DATABASE']
    return db unless db

    db.each do |e|
      db = {'NAME' => nil, 'NOTE' => nil, 'WWW' => nil, 'FTP' => nil}
      e.sub(/.$/,'').split(/;/).each do |line|
        case line
        when /NAME=(.+)/
          db['NAME'] = $1
        when /NOTE=(.+)/
          db['NOTE'] = $1
        when /WWW="(.+)"/
          db['WWW'] = $1
        when /FTP="(.+)"/
          db['FTP'] = $1
        end 
      end
      tmp.push(db)
    end
    return tmp
  when nil
    return @data['CC']
  else
    return @data['CC'][topic]
  end
end

#dr(key = nil) ⇒ Object

Bio::UniProtKB#dr



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# File 'lib/bio/db/embl/uniprotkb.rb', line 1131

def dr(key = nil)
  unless key
    embl_dr
  else
    (embl_dr[key] or []).map {|x|
      {'Accession' => x[0],
       'Version' => x[1],
       ' ' => x[2],
       'Molecular Type' => x[3]}
    }
  end
end

#dt(key = nil) ⇒ Object

returns a Hash of information in the DT lines.

hash keys: 
  ['created', 'sequence', 'annotation']

–

also Symbols acceptable (ASAP):
  [:created, :sequence, :annotation]

++

Since UniProtKB release 7.0 of 07-Feb-2006, the DT line format is changed, and the word “annotation” is no longer used in DT lines. Despite the change, the word “annotation” is still used for keeping compatibility.

returns a String of information in the DT lines by a given key.

DT Line; date (3/entry)

DT DD-MMM-YYY (integrated into UniProtKB/XXXXX.)
DT DD-MMM-YYY (sequence version NN)
DT DD-MMM-YYY (entry version NN)

The format have been changed in UniProtKB release 7.0 of 07-Feb-2006. Below is the older format.

Old format of DT Line; date (3/entry)

DT DD-MMM-YYY (rel. NN, Created)
DT DD-MMM-YYY (rel. NN, Last sequence update)
DT DD-MMM-YYY (rel. NN, Last annotation update)



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# File 'lib/bio/db/embl/uniprotkb.rb', line 157

def dt(key = nil)
  return dt[key] if key
  return @data['DT'] if @data['DT']

  part = self.get('DT').split(/\n/)
  @data['DT'] = {
    'created'    => part[0].sub(/\w{2}   /,'').strip,
    'sequence'   => part[1].sub(/\w{2}   /,'').strip,
    'annotation' => part[2].sub(/\w{2}   /,'').strip
  }
end

#embl_dr ⇒ Object

Backup Bio::EMBLDB#dr as embl_dr



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# File 'lib/bio/db/embl/uniprotkb.rb', line 1128

alias :embl_dr :dr

#entry_id ⇒ Object Also known as: entry_name, entry

returns a ENTRY_NAME in the ID line.



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# File 'lib/bio/db/embl/uniprotkb.rb', line 98

def entry_id
  id_line('ENTRY_NAME')
end

#ft(feature_key = nil) ⇒ Object

returns contents in the feature table.

Examples

sp = Bio::UniProtKB.new(entry)
ft = sp.ft
ft.class #=> Hash
ft.keys.each do |feature_key|
  ft[feature_key].each do |feature|
    feature['From'] #=> '1'
    feature['To']   #=> '21'
    feature['Description'] #=> ''
    feature['FTId'] #=> ''
    feature['diff'] #=> []
    feature['original'] #=> [feature_key, '1', '21', '', '']
  end
end

  • Bio::UniProtKB#ft -> Hash

    {FEATURE_KEY => [{'From' => int, 'To' => int, 
                  'Description' => aStr, 'FTId' => aStr,
                  'diff' => [original_residues, changed_residues],
                  'original' => aAry }],...}

returns an Array of the information about the feature_name in the feature table.

  • Bio::UniProtKB#ft(feature_name) -> Array of Hash

    [{'From' => str, 'To' => str, 'Description' => str, 'FTId' => str},...]

FT Line; feature table data (>=0, optional)

Col     Data item
-----   -----------------
 1- 2   FT
 6-13   Feature name 
15-20   `FROM' endpoint
22-27   `TO' endpoint
35-75   Description (>=0 per key)
-----   -----------------

Note: ‘FROM’ and ‘TO’ endopoints are allowed to use non-numerial charactors including ‘<’, ‘>’ or ‘?’. (c.f. ‘<1’, ‘?42’)

See also www.expasy.org/sprot/userman.html#FT_line



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# File 'lib/bio/db/embl/uniprotkb.rb', line 1196

def ft(feature_key = nil)
  return ft[feature_key] if feature_key
  return @data['FT'] if @data['FT']

  table = []
  begin
    get('FT').split("\n").each do |line|
      if line =~ /^FT   \w/
        feature = line.chomp.ljust(74)
        table << [feature[ 5..12].strip,   # Feature Name
                  feature[14..19].strip,   # From
                  feature[21..26].strip,   # To
                  feature[34..74].strip ]  # Description
      else
        table.last << line.chomp.sub!(/^FT +/, '')
      end
    end

    # Joining Description lines
    table = table.map { |feature| 
      ftid = feature.pop if feature.last =~ /FTId=/
      if feature.size > 4
        feature = [feature[0], 
                   feature[1], 
                   feature[2], 
                   feature[3, feature.size - 3].join(" ")]
      end
      feature << if ftid then ftid else '' end
    }

    hash = {}
    table.each do |feature|
      hash[feature[0]] = [] unless hash[feature[0]]
      hash[feature[0]] << {
        # Removing '<', '>' or '?' in FROM/TO endopoint.
        'From' => feature[1].sub(/\D/, '').to_i,  
        'To'   => feature[2].sub(/\D/, '').to_i, 
        'Description' => feature[3], 
        'FTId' => feature[4].to_s.sub(/\/FTId=/, '').sub(/\.$/, ''),
        'diff' => [],
        'original' => feature
      }

      case feature[0]
      when 'VARSPLIC', 'VARIANT', 'VAR_SEQ', 'CONFLICT'
        case hash[feature[0]].last['Description']
        when /(\w[\w ]*\w*) - ?> (\w[\w ]*\w*)/
          original_res = $1
          changed_res = $2
          original_res = original_res.gsub(/ /,'').strip
          chenged_res = changed_res.gsub(/ /,'').strip
        when /Missing/i
          original_res = seq.subseq(hash[feature[0]].last['From'],
                                    hash[feature[0]].last['To'])
          changed_res = ''
        end
        hash[feature[0]].last['diff'] = [original_res, chenged_res]
      end
    end
  rescue
    raise "Invalid FT Lines(#{$!}) in #{entry_id}:, \n'#{self.get('FT')}'\n"
  end

  @data['FT'] = hash
end

#gene_name ⇒ Object

returns a String of the first gene name in the GN line.



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# File 'lib/bio/db/embl/uniprotkb.rb', line 437

def gene_name
  (x = self.gene_names) ? x.first : nil
end

#gene_names ⇒ Object

returns a Array of gene names in the GN line.



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# File 'lib/bio/db/embl/uniprotkb.rb', line 426

def gene_names
  gn # set @data['GN'] if it hasn't been already done
  if @data['GN'].first.class == Hash then
    @data['GN'].collect { |element| element[:name] }
  else
    @data['GN'].first
  end
end

#gn ⇒ Object

returns gene names in the GN line.

New UniProt/SwissProt format:

  • Bio::UniProtKB#gn -> [ <gene record>* ]

where <gene record> is:

{ :name => '...', 
  :synonyms => [ 's1', 's2', ... ],
  :loci   => [ 'l1', 'l2', ... ],
  :orfs     => [ 'o1', 'o2', ... ] 
}

Old format:

GN Line: Gene name(s) (>=0, optional)



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# File 'lib/bio/db/embl/uniprotkb.rb', line 350

def gn
  unless @data['GN']
    case fetch('GN')
    when /Name=/,/ORFNames=/,/OrderedLocusNames=/,/Synonyms=/
      @data['GN'] = gn_uniprot_parser
    else
      @data['GN'] = gn_old_parser
    end
  end
  @data['GN']
end

#hi ⇒ Object

The HI line

Bio::UniProtKB#hi #=> hash



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# File 'lib/bio/db/embl/uniprotkb.rb', line 690

def hi
  unless @data['HI']
    @data['HI'] = []
    fetch('HI').split(/\. /).each do |hlist|
      hash = {'Category' => '',  'Keywords' => [], 'Keyword' => ''}
      hash['Category'], hash['Keywords'] = hlist.split(': ')
      hash['Keywords'] = hash['Keywords'].split('; ')
      hash['Keyword'] = hash['Keywords'].pop
      hash['Keyword'].sub!(/\.$/, '')
      @data['HI'] << hash
    end
  end
  @data['HI']
end

#id_line(key = nil) ⇒ Object

returns a Hash of the ID line.

returns a content (Int or String) of the ID line by a given key. Hash keys: [‘ENTRY_NAME’, ‘DATA_CLASS’, ‘MODECULE_TYPE’, ‘SEQUENCE_LENGTH’]

ID Line (since UniProtKB release 9.0 of 31-Oct-2006)

ID   P53_HUMAN               Reviewed;         393 AA.
#"ID  #{ENTRY_NAME} #{DATA_CLASS}; #{SEQUENCE_LENGTH}."

Examples

obj.id_line  #=> {"ENTRY_NAME"=>"P53_HUMAN", "DATA_CLASS"=>"Reviewed", 
                  "SEQUENCE_LENGTH"=>393, "MOLECULE_TYPE"=>nil}

obj.id_line('ENTRY_NAME') #=> "P53_HUMAN"

ID Line (older style)

ID   P53_HUMAN      STANDARD;      PRT;   393 AA.
#"ID  #{ENTRY_NAME} #{DATA_CLASS}; #{MOLECULE_TYPE}; #{SEQUENCE_LENGTH}."

Examples

obj.id_line  #=> {"ENTRY_NAME"=>"P53_HUMAN", "DATA_CLASS"=>"STANDARD", 
                  "SEQUENCE_LENGTH"=>393, "MOLECULE_TYPE"=>"PRT"}

obj.id_line('ENTRY_NAME') #=> "P53_HUMAN"



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# File 'lib/bio/db/embl/uniprotkb.rb', line 73

def id_line(key = nil)
  return id_line[key] if key
  return @data['ID'] if @data['ID']

  part = @orig['ID'].split(/ +/)         
  if part[4].to_s.chomp == 'AA.' then
    # after UniProtKB release 9.0 of 31-Oct-2006
    # (http://www.uniprot.org/docs/sp_news.htm)
    molecule_type   = nil
    sequence_length = part[3].to_i
  else
    molecule_type   = part[3].sub(/;/,'')
    sequence_length = part[4].to_i
  end
  @data['ID'] = {
    'ENTRY_NAME'      => part[1],
    'DATA_CLASS'      => part[2].sub(/;/,''),
    'MOLECULE_TYPE'   => molecule_type,
    'SEQUENCE_LENGTH' => sequence_length
  }
end

#molecule ⇒ Object Also known as: molecule_type

returns a MOLECULE_TYPE in the ID line.

A short-cut for Bio::UniProtKB#id_line(‘MOLECULE_TYPE’).



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# File 'lib/bio/db/embl/uniprotkb.rb', line 108

def molecule
  id_line('MOLECULE_TYPE')
end

#oh ⇒ Object

The OH Line;

OH NCBI_TaxID=TaxID; HostName. br.expasy.org/sprot/userman.html#OH_line



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# File 'lib/bio/db/embl/uniprotkb.rb', line 520

def oh
  unless @data['OH']
    @data['OH'] = fetch('OH').split("\. ").map {|x|
      if x =~ /NCBI_TaxID=(\d+);/
        taxid = $1
      else
        raise ArgumentError, ["Error: Invalid OH line format (#{self.entry_id}):",
                              $!, "\n", get('OH'), "\n"].join
        
      end
      if x =~ /NCBI_TaxID=\d+; (.+)/ 
        host_name = $1
        host_name.sub!(/\.$/, '')
      else
        host_name = nil
      end
      {'NCBI_TaxID' => taxid, 'HostName' => host_name}
    }
  end
  @data['OH']
end

#os(num = nil) ⇒ Object

returns a Array of Hashs or a String of the OS line when a key given.

  • Bio::EMBLDB#os -> Array

[{'name' => '(Human)', 'os' => 'Homo sapiens'}, 
 {'name' => '(Rat)', 'os' => 'Rattus norveticus'}]

{'name' => "(Human)", 'os' => 'Homo sapiens'}

  • Bio::UniProtKB#os[‘name’] -> “(Human)”

  • Bio::EPTR#os(0) -> “Homo sapiens (Human)”

OS Line; organism species (>=1)

OS   Genus species (name).
OS   Genus species (name0) (name1).
OS   Genus species (name0) (name1).
OS   Genus species (name0), G s0 (name0), and G s (name0) (name1).
OS   Homo sapiens (Human), and Rarrus norveticus (Rat)
OS   Hippotis sp. Clark and Watts 825.
OS   unknown cyperaceous sp.



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# File 'lib/bio/db/embl/uniprotkb.rb', line 459

def os(num = nil)
  unless @data['OS']
    os = Array.new
    fetch('OS').split(/, and|, /).each do |tmp|
      if tmp =~ /(\w+ *[\w \:\'\+\-\.]+[\w\.])/
        org = $1
        tmp =~ /(\(.+\))/ 
        os.push({'name' => $1, 'os' => org})
      else
        raise "Error: OS Line. #{$!}\n#{fetch('OS')}\n"
      end
    end
    @data['OS'] = os
  end

  if num
    # EX. "Trifolium repens (white clover)"
    return "#{@data['OS'][num]['os']} #{@data['OS'][num]['name']}"
  else
    return @data['OS']
  end
end

#ox ⇒ Object

returns a Hash of oraganism taxonomy cross-references.

  • Bio::UniProtKB#ox -> Hash

    {'NCBI_TaxID' => ['1234','2345','3456','4567'], ...}

OX Line; organism taxonomy cross-reference (>=1 per entry)

OX   NCBI_TaxID=1234;
OX   NCBI_TaxID=1234, 2345, 3456, 4567;



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# File 'lib/bio/db/embl/uniprotkb.rb', line 503

def ox
  unless @data['OX']
    tmp = fetch('OX').sub(/\.$/,'').split(/;/).map { |e| e.strip }
    hsh = Hash.new
    tmp.each do |e|
      db,refs = e.split(/=/)
      hsh[db] = refs.split(/, */)
    end
    @data['OX'] = hsh
  end
  return @data['OX']
end

#protein_name ⇒ Object

returns the proposed official name of the protein. Returns a String.

Since UniProtKB release 14.0 of 22-Jul-2008, the DE line format have been changed. The method returns the full name which is taken from “RecName: Full=” or “SubName: Full=” line normally in the beginning of the DE lines. Unlike parser for old format, no special treatments for fragment or precursor.

For old format, the method parses the DE lines and returns the protein name as a String.

DE Line; description (>=1)

"DE #{OFFICIAL_NAME} (#{SYNONYM})"
"DE #{OFFICIAL_NAME} (#{SYNONYM}) [CONTEINS: #1; #2]."
OFFICIAL_NAME  1/entry
SYNONYM        >=0
CONTEINS       >=0



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# File 'lib/bio/db/embl/uniprotkb.rb', line 250

def protein_name
  @data['DE'] ||= parse_DE_line_rel14(get('DE'))
  parsed_de_line = @data['DE']
  if parsed_de_line then
    # since UniProtKB release 14.0 of 22-Jul-2008
    name = nil
    parsed_de_line.each do |a|
      case a[0]
      when 'RecName', 'SubName'
        if name_pair = a[1..-1].find { |b| b[0] == 'Full' } then
          name = name_pair[1]
          break
        end
      end
    end
    name = name.to_s
  else
    # old format (before Rel. 13.x)
    name = ""
    if de_line = fetch('DE') then
      str = de_line[/^[^\[]*/] # everything preceding the first [ (the "contains" part)
      name = str[/^[^(]*/].strip
      name << ' (Fragment)' if str =~ /fragment/i
    end
  end
  return name
end

#ref ⇒ Object

returns contents in the R lines.

  • Bio::EMBLDB::Common#ref -> [ <refernece information Hash>* ]

where <reference information Hash> is:

{'RN' => '', 'RC' => '', 'RP' => '', 'RX' => '', 
 'RA' => '', 'RT' => '', 'RL' => '', 'RG' => ''}

R Lines

  • RN RC RP RX RA RT RL RG



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# File 'lib/bio/db/embl/uniprotkb.rb', line 556

def ref
  unless @data['R']
    @data['R'] = [get('R').split(/\nRN   /)].flatten.map { |str|
      hash = {'RN' => '', 'RC' => '', 'RP' => '', 'RX' => '', 
             'RA' => '', 'RT' => '', 'RL' => '', 'RG' => ''}
      str = 'RN   ' + str unless /^RN   / =~ str

      str.split("\n").each do |line|
        if /^(R[NPXARLCTG])   (.+)/ =~ line
          hash[$1] += $2 + ' '
        else
          raise "Invalid format in R lines, \n[#{line}]\n"
        end
      end

      hash['RN'] = set_RN(hash['RN'])
      hash['RC'] = set_RC(hash['RC'])
      hash['RP'] = set_RP(hash['RP'])
      hash['RX'] = set_RX(hash['RX'])
      hash['RA'] = set_RA(hash['RA'])
      hash['RT'] = set_RT(hash['RT'])
      hash['RL'] = set_RL(hash['RL'])
      hash['RG'] = set_RG(hash['RG'])

      hash
    }

  end
  @data['R']
end

#references ⇒ Object

returns Bio::Reference object from Bio::EMBLDB::Common#ref.

  • Bio::EMBLDB::Common#ref -> Bio::References



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# File 'lib/bio/db/embl/uniprotkb.rb', line 650

def references
  unless @data['references']
    ary = self.ref.map {|ent|
      hash = Hash.new('')
      ent.each {|key, value|
        case key
        when 'RA'
          hash['authors'] = value.split(/, /)
        when 'RT'
          hash['title'] = value
        when 'RL'
          if value =~ /(.*) (\d+) \((\d+)\), (\d+-\d+) \((\d+)\)$/
            hash['journal'] = $1
            hash['volume']  = $2
            hash['issue']   = $3
            hash['pages']   = $4
            hash['year']    = $5
          else
            hash['journal'] = value
          end
        when 'RX'  # PUBMED, MEDLINE, DOI
          value.each do |tag, xref|
            hash[ tag.downcase ]  = xref
          end
        end
      }
      Reference.new(hash)
    }
    @data['references'] = References.new(ary)
  end
  @data['references']
end

#seq ⇒ Object Also known as: aaseq

returns a Bio::Sequence::AA of the amino acid sequence.

  • Bio::UniProtKB#seq -> Bio::Sequence::AA

blank Line; sequence data (>=1)



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# File 'lib/bio/db/embl/uniprotkb.rb', line 1306

def seq
  unless @data['']
    @data[''] = Sequence::AA.new( fetch('').gsub(/ |\d+/,'') )
  end
  return @data['']
end

#sequence_length ⇒ Object Also known as: aalen

returns a SEQUENCE_LENGTH in the ID line.

A short-cut for Bio::UniProtKB#id_line(‘SEQUENCE_LENGHT’).



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# File 'lib/bio/db/embl/uniprotkb.rb', line 117

def sequence_length
  id_line('SEQUENCE_LENGTH')
end

#set_RN(data) ⇒ Object 



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# File 'lib/bio/db/embl/uniprotkb.rb', line 587

def set_RN(data)
  data.strip
end

#sq(key = nil) ⇒ Object

returns a Hash of conteins in the SQ lines.

  • Bio::UniProtKBL#sq -> hsh

returns a value of a key given in the SQ lines.

  • Bio::UniProtKBL#sq(key) -> int or str

  • Keys: [‘MW’, ‘mw’, ‘molecular’, ‘weight’, ‘aalen’, ‘len’, ‘length’, 

    'CRC64']

SQ Line; sequence header (1/entry)

SQ   SEQUENCE   233 AA;  25630 MW;  146A1B48A1475C86 CRC64;
SQ   SEQUENCE  \d+ AA; \d+ MW;  [0-9A-Z]+ CRC64;

MW, Dalton unit. CRC64 (64-bit Cyclic Redundancy Check, ISO 3309).



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# File 'lib/bio/db/embl/uniprotkb.rb', line 1278

def sq(key = nil)
  unless @data['SQ']
    if fetch('SQ') =~ /(\d+) AA\; (\d+) MW; (.+) CRC64;/
      @data['SQ'] = { 'aalen' => $1.to_i, 'MW' => $2.to_i, 'CRC64' => $3 }
    else
      raise "Invalid SQ Line: \n'#{fetch('SQ')}'"
    end
  end

  if key
    case key
    when /mw/, /molecular/, /weight/
      @data['SQ']['MW']
    when /len/, /length/, /AA/
      @data['SQ']['aalen']
    else
      @data['SQ'][key]
    end
  else 
    @data['SQ']
  end
end

#synonyms ⇒ Object

returns synonyms (unofficial and/or alternative names). Returns an Array containing String objects.

Since UniProtKB release 14.0 of 22-Jul-2008, the DE line format have been changed. The method returns the full or short names which are taken from “RecName: Short=”, “RecName: EC=”, and AltName lines, except after “Contains:” or “Includes:”. For keeping compatibility with old format parser, “RecName: EC=N.N.N.N” is reported as “EC N.N.N.N”. In addition, to prevent confusion, “Allergen=” and “CD_antigen=” prefixes are added for the corresponding fields.

For old format, the method parses the DE lines and returns synonyms. synonyms are each placed in () following the official name on the DE line.



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# File 'lib/bio/db/embl/uniprotkb.rb', line 293

def synonyms
  ary = Array.new
  @data['DE'] ||= parse_DE_line_rel14(get('DE'))
  parsed_de_line = @data['DE']
  if parsed_de_line then
    # since UniProtKB release 14.0 of 22-Jul-2008
    parsed_de_line.each do |a|
      case a[0]
      when 'Includes', 'Contains'
        break #the each loop
      when 'RecName', 'SubName', 'AltName'
        a[1..-1].each do |b|
          if name = b[1] and b[1] != self.protein_name then
            case b[0]
            when 'EC'
              name = "EC " + b[1]
            when 'Allergen', 'CD_antigen'
              name = b[0] + '=' + b[1]
            else
              name = b[1]
            end
            ary.push name
          end
        end
      end #case a[0]
    end #parsed_de_line.each
  else
    # old format (before Rel. 13.x)
    if de_line = fetch('DE') then
      line = de_line.sub(/\[.*\]/,'') # ignore stuff between [ and ].  That's the "contains" part
    line.scan(/\([^)]+/) do |synonym| 
      unless synonym =~ /fragment/i then 
        ary << synonym[1..-1].strip # index to remove the leading (  
      end
      end
    end
  end
  return ary
end