Class: ScanForRestrSite

Inherits:
Object
  • Object
show all
Defined in:
lib/seqtrimnext/classes/scan_for_restr_site.rb

Overview

Author

Almudena Bocinos Rioboo

This class provided the methods to read the parameter’s file and to create the structure where will be storaged the param’s name and the param’s numeric-value

Instance Method Summary collapse

Constructor Details

#initialize(sequence, rest) ⇒ ScanForRestrSite

Creates the structure and start the reading of parameter’s file



10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
# File 'lib/seqtrimnext/classes/scan_for_restr_site.rb', line 10

def initialize(sequence,rest)    
  @seq_fasta=sequence 
  @rest=rest      
  puts "#{@seq_fasta}  , #{@rest}"  
  res = execute
  

  res.each do |e| 
      puts "#{e.join(',')}"
  end
  
# selects from res,the max good hit  
  puts "--- MAX: --- "
  
  max = res.max{|e1,e2| e1[1]<=> e2[1]}
                    
  puts max.join(' ; ')                    
                                           
# checks if the max one has the size of restriction with a margen error
  margen = (@rest.size <= 4)? 0 : 1;  # <- don't change       
  if ((max[1] !=  @rest.size) && (max[1] != @rest.size-margen)) 
    puts "-the max good hit hasn't the size minimum: #{@rest.size} or #{@rest.size-margen} "
    max=[]
  end
  
  
  
  #read_file(path)   
end

Instance Method Details

#executeObject



42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
# File 'lib/seqtrimnext/classes/scan_for_restr_site.rb', line 42

def execute    
  r=[]
                            # 
                            # for (my $p=0; $p < $sL-$srfL; $p++){
                            #     $os = $ns = $xs = 0;
                            #     for ( my $i=0; $i < $srfL; $i++ ) {
                            #         my $c  = substr($s, $i+$p, 1);  # ver si decrementar antes pos
                            #         my $cc = substr($restrSite, $i, 1);
                            #         if ($c eq $cc) {
                            #             ++$os;
                            #         } elsif ($c eq "N"){
                            #             ++$ns;
                            #         } else {
                            #             ++$xs;
                            #         }
                            #     }
                            #     $r[$p] = [$p, $os, $ns, $xs];
                            #         print "$p, $os, $ns, $xs\n";
                            # }                     
  for p in 0..@seq_fasta.size-@rest.size 
      os = 0; 
      ns = 0; 
      xs = 0;                    
      puts "-------[#{p}]-#{@seq_fasta[p,@seq_fasta.size-p]}  , #{@rest}"  
      
     i=0
    @rest.each_char do |cc|
       c = @seq_fasta[i+p].chr
       puts "(#{c}==#{cc})=>#{c==cc}"
      if (c == cc)
        os += 1
      elsif (c == 'N')
        ns += 1
      else     
        xs += 1
      end
      i+=1     
      
    end
    r[p]=[p,os,ns,xs]
     puts r[p].join(',')
  end     
  return r
end

#exists?(param_name) ⇒ Boolean

Returns true if exists the parameter and nil if don’t

Returns:

  • (Boolean)


131
132
133
# File 'lib/seqtrimnext/classes/scan_for_restr_site.rb', line 131

def exists?(param_name)
  return !@h[param_name].nil?
end

#get_param(param) ⇒ Object

Return the parameter’s list in an array



119
120
121
122
# File 'lib/seqtrimnext/classes/scan_for_restr_site.rb', line 119

def get_param(param)
  #$LOG.debug "Get Param:  #{@h[param]}"
  return @h[param]
end

Prints the pair name/numeric-value for every parameter



111
112
113
114
115
116
# File 'lib/seqtrimnext/classes/scan_for_restr_site.rb', line 111

def print_parameters()
  @h.each do |clave, valor|
    
    $LOG.debug  "The Parameter #{clave} have the value " +valor.to_s
  end
end

#read_file(path_fichero) ⇒ Object

Reads param’s file



88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
# File 'lib/seqtrimnext/classes/scan_for_restr_site.rb', line 88

def read_file(path_fichero)
  File.open(path_fichero).each_line do |line| 
         
    line.chomp! # delete end of line      
    
    if !line.empty?
      if !(line =~ /^#/)   # if line is not a comment
        # extract the parameter's name in params[0] and the parameter's value in params[1]
        params = line.split(/\s*=\s*/)
        
        # storage in the hash the pair key/value, in our case will be name/numeric-value , 
        # that are save in params[0] and params[1],  respectively
        @h[params[0]] = params[1]
        
        $LOG.debug "read: #{params[1]}"
      end # end if comentario
    end #end if line
  end #end each
  $LOG.info "File Params have been readed"

end

#set_param(param, value) ⇒ Object



124
125
126
# File 'lib/seqtrimnext/classes/scan_for_restr_site.rb', line 124

def set_param(param,value)
  @h[param] = value
end