Class: OpenTrons::Pipette

Inherits:
Object
  • Object
show all
Defined in:
lib/opentrons/instruments.rb

Direct Known Subclasses

MultiPipette

Instance Attribute Summary collapse

Instance Method Summary collapse

Constructor Details

#initialize(protocol, instruments, model, mount: "left", tip_racks: [], tip_model: nil) ⇒ Pipette 



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# File 'lib/opentrons/instruments.rb', line 77

def initialize(protocol, instruments, model, mount: "left", tip_racks: [], tip_model: nil)
  @protocol = protocol
  @instruments = instruments
  @model = model

  @mount = mount
  @tip_racks = tip_racks
  @tip_model = tip_model
end

Instance Attribute Details

#instruments ⇒ Object

Returns the value of attribute instruments.



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# File 'lib/opentrons/instruments.rb', line 75

def instruments
  @instruments
end

#model ⇒ Object

Returns the value of attribute model.



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# File 'lib/opentrons/instruments.rb', line 75

def model
  @model
end

#mount ⇒ Object

Returns the value of attribute mount.



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# File 'lib/opentrons/instruments.rb', line 75

def mount
  @mount
end

#protocol ⇒ Object

Returns the value of attribute protocol.



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# File 'lib/opentrons/instruments.rb', line 75

def protocol
  @protocol
end

#tip_model ⇒ Object

Returns the value of attribute tip_model.



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# File 'lib/opentrons/instruments.rb', line 75

def tip_model
  @tip_model
end

#tip_racks ⇒ Object

Returns the value of attribute tip_racks.



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# File 'lib/opentrons/instruments.rb', line 75

def tip_racks
  @tip_racks
end

Instance Method Details

#aspirate(volume, location) ⇒ Object 



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# File 'lib/opentrons/instruments.rb', line 102

def aspirate(volume, location)
  command = Aspirate.new(self, volume, location)
  protocol.commands.command_list << command
  return command
end

#blowout(location) ⇒ Object 



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# File 'lib/opentrons/instruments.rb', line 193

def blowout(location)
  command = Blowout.new(self, location)
  protocol.commands.command_list << command
  return command
end

#delay(wait, message: "") ⇒ Object 



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# File 'lib/opentrons/instruments.rb', line 199

def delay(wait, message: "")
  command = Delay.new(wait, message)
  protocol.commands.command_list << command
  return command
end

#dispense(volume, location) ⇒ Object 



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# File 'lib/opentrons/instruments.rb', line 108

def dispense(volume, location)
  command = Dispense.new(self, volume, location)
  protocol.commands.command_list << command
  return command
end

#drop_tip(location = false) ⇒ Object 



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# File 'lib/opentrons/instruments.rb', line 180

def drop_tip(location=false)
  location = protocol.trash.wells(0) if !location
  command = DropTip.new(self, location)
  protocol.commands.command_list << command
  return command
end

#get_next_tip(multi: false) ⇒ Object

For whatever reason, air gap has no location in Python API but has a location in the JSON schema. Not implemented for now. def air_gap(volume, location) command = Aspirate.new(self, volume, location) self.protocol.commands << command return command end



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# File 'lib/opentrons/instruments.rb', line 122

def get_next_tip(multi: false)
  location = nil
  catch :tip_found do
    tip_racks.each do |tip_rack|
      tip_rack.well_list.each do |column|
        if multi
          if column.all? {|x| x.tip}
            location = column[0]
            throw :tip_found
          end
        else
          column.each do |x|
            if x.tip
              location = x
              throw :tip_found
            end
          end
        end
      end
    end
    return false
  end

  return location
end

#inspect ⇒ Object 



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# File 'lib/opentrons/instruments.rb', line 98

def inspect
  to_s
end

#pick_up_tip(location = false) ⇒ Object 



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# File 'lib/opentrons/instruments.rb', line 148

def pick_up_tip(location=false)
  if !location
    tip_location = self.get_next_tip()
    # If no tip found and tip model is provided, create a tip rack.

    if !tip_location
      if tip_model
        tip_racks << protocol.labware.load(tip_model, protocol.labware.free_slots[-1], 'Auto-generated-tip-rack')
      else
        raise ArgumentError.new "pick_up_tip called without location and pipette is out of tips."
      end
    end
    tip_location = self.get_next_tip()
    location = tip_location
  end

  if location.is_a? Array
    well = location[0]
  else
    well = location
  end

  if !(well.tip)
    puts "Warning: Already picked up tip at #{location}."
  else
    well.tip = false
  end

  command = PickUpTip.new(self, location)
  protocol.commands.command_list << command
  return command
end

#to_hash ⇒ Object 



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# File 'lib/opentrons/instruments.rb', line 87

def to_hash
  as_hash = {}
  as_hash["mount"] = mount
  as_hash["model"] = model
  return as_hash
end

#to_s ⇒ Object 



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# File 'lib/opentrons/instruments.rb', line 94

def to_s
  "<OpenTrons::Pipette:0x#{self.__id__.to_s(16)}>"
end

#touch_tip(location) ⇒ Object 



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# File 'lib/opentrons/instruments.rb', line 187

def touch_tip(location)
  command = TouchTip.new(self, location)
  protocol.commands.command_list << command
  return command
end