Class: Bioroebe::ConsensusSequence
Overview
Bioroebe::ConsensusSequence
Constant Summary
Bioroebe::CommandlineApplication::OLD_VERBOSE_VALUE
Bioroebe::ColoursForBase::ARRAY_HTML_COLOURS_IN_USE
Constants inherited
from Base
Base::NAMESPACE
Instance Method Summary
collapse
#all_aminoacids?, #append_what_into, #at_home?, #be_silent, #be_verbose?, #cat, #ccliner, #change_directory, #cliner, #codon_table_dataset?, #codon_to_aminoacid, #codons_for?, #colourize_this_dna_sequence, #complement, #cp, #disable_warnings, #download_dir?, #editor?, #enable_warnings, #ensure_that_the_base_directories_exist, #esystem, #extract, #is_this_a_start_codon?, #is_this_a_stop_codon?, #leading_five_prime, #load_bioroebe_yaml_file, #log_directory?, #one_letter_to_long_name, #one_to_three, #only_numbers?, #open_in_browser, #opne, #opnn, #pad_with_double_quotes, #pad_with_single_quotes, #partner_nucleotide, #remove_numbers, #remove_trailing_ansii_escape_code, #return_all_possible_start_codons, #return_array_of_one_letter_aminoacids, #return_cheerful_person, #return_chunked_display, #return_ubiquitin_sequence, #set_be_verbose, #start_codon?, #stop_codons?, #strict_filter_away_invalid_aminoacids, #taxonomy_download_directory?, #three_to_one, #to_rna, #trailing_three_prime, #use_opn?, #was_or_were, #without_extname, #write_what_into
#commandline_arguments?, #commandline_arguments_that_are_files?, #e, #first?, #first_non_hyphen_argument?, #remove_hyphens_from_the_commandline_arguments, #return_commandline_arguments_as_string, #return_commandline_arguments_that_are_not_files, #return_entries_without_two_leading_hyphens, #select_commandline_arguments, #select_entries_starting_with_two_hyphens, #set_commandline_arguments
#colourize_this_aminoacid_sequence_for_the_commandline, #colourize_this_nucleotide_sequence, #disable_colours, #ecomment, #egold, #enable_colours, #eorange, #eparse, #erev, #red, #remove_trailing_escape_part, #return_colour_for_nucleotides, #rev, #sdir, #set_use_colours, #sfancy, #sfile, #simp, #swarn, #use_colours?, #use_colours_within_the_bioroebe_namespace?
Methods inherited from Base
#append_what_into, #can_base_pair?, #convert_global_env, #delete_file, #directory_to_the_codon_tables?, #file_readlines, #is_on_roebe?, #is_palindrome?, #main_encoding?, #mkdir, #move_file, #mv, #no_file_exists_at, #no_newlines, #project_yaml_directory?, #rds, #register_sigint, #return_pwd, #write_what_into
Constructor Details
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# File 'lib/bioroebe/utility_scripts/consensus_sequence.rb', line 24
def initialize(*i)
reset
run_already = true if block_given?
yielded = yield
case yielded when :do_not_run_yet_and_be_quiet
run_already = false
@be_verbose = false
@report_the_frequencies = false
when :do_not_run_yet
run_already = false
when :be_quiet
@be_verbose = false
@report_the_frequencies = false
end
end
set_input_sequences(i)
run if run_already
end
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Instance Method Details
#calculate_frequencies_of(array) ⇒ Object
#
calculate_frequencies_of
#
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# File 'lib/bioroebe/utility_scripts/consensus_sequence.rb', line 185
def calculate_frequencies_of(array)
hash = {}
hash['A'] = array.count('A')
hash['T'] = array.count('T')
hash['C'] = array.count('C')
hash['G'] = array.count('G')
return hash
end
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#colourize_nucleotide_position(i) ⇒ Object
#
colourize_nucleotide_position
#
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# File 'lib/bioroebe/utility_scripts/consensus_sequence.rb', line 137
def colourize_nucleotide_position(i)
seagreen(i)
end
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#consensus_sequence? ⇒ Boolean
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# File 'lib/bioroebe/utility_scripts/consensus_sequence.rb', line 178
def consensus_sequence?
@array_consensus_positions.join
end
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#display_an_even_simpler_consensus_sequence(i = consensus_sequence?
) ⇒ Object
#
display_an_even_simpler_consensus_sequence
#
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# File 'lib/bioroebe/utility_scripts/consensus_sequence.rb', line 255
def display_an_even_simpler_consensus_sequence(
i = consensus_sequence?
)
this_sequence = orange(return_an_even_simpler_consensus_sequence(i))
erev " #{this_sequence}#{rev} "\
"# ← a very likely consensus sequence"
end
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# File 'lib/bioroebe/utility_scripts/consensus_sequence.rb', line 79
def input_sequences?
@input_sequences
end
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#iterate_over_the_sequences ⇒ Object
Also known as:
determine_the_consensus_sequences
#
iterate_over_the_sequences
#
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# File 'lib/bioroebe/utility_scripts/consensus_sequence.rb', line 93
def iterate_over_the_sequences
size = @input_sequences.first.size
0.upto(size - 1) {|n|
mapped = @input_sequences.map {|line| line[n,1] }
frequencies = calculate_frequencies_of(mapped)
max_value = frequencies.group_by {|key, value| value }.max.last _ = max_value.map {|first, last| first }
if _.size > 1
append_this = '['+_.join('/')+']'
else
append_this = _.join
end
if @report_the_frequencies
erev "The #{mediumslateblue('frequencies')}#{rev} "\
"for #{lightgreen(mapped.join)}#{rev} "+
'(at '+colourize_nucleotide_position("nucleotide position #{n+1}")+
rev+') were:'
e
print ' '; pp frequencies
erev "\nThe associated max-value for "+
colourize_nucleotide_position("nucleotide position #{n+1}")+
"#{rev} was: "+
simp(
max_value.map {|a| a.join(': ') }.join('; ')
)
e
end
@array_consensus_positions << append_this
}
end
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#random_nucleotide_for_this_IUPAC_code(i) ⇒ Object
#
random_nucleotide_for_this_IUPAC_code
#
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# File 'lib/bioroebe/utility_scripts/consensus_sequence.rb', line 144
def random_nucleotide_for_this_IUPAC_code(i)
case i
when 'H'
return_A_or_T_or_C
when 'M'
return_A_or_C
end
end
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#report_on_which_sequences_we_will_operate(be_verbose = @be_verbose) ⇒ Object
#
report_on_which_sequences_we_will_operate
#
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# File 'lib/bioroebe/utility_scripts/consensus_sequence.rb', line 197
def report_on_which_sequences_we_will_operate(
be_verbose = @be_verbose
)
case be_verbose
when :be_verbose
be_verbose = true
end
if be_verbose
e
erev "#{rev}Working on the following "\
"#{steelblue(@input_sequences.size)} "\
"#{rev}sequences next:"
e
@input_sequences.each_with_index {|this_sequence, index| index += 1
e " #{sfancy(this_sequence)} #{steelblue(index.to_s.rjust(3))}"
}; e
end
end
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#report_the_consensus_sequence ⇒ Object
#
report_the_consensus_sequence
#
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# File 'lib/bioroebe/utility_scripts/consensus_sequence.rb', line 219
def report_the_consensus_sequence
if @be_verbose
the_consensus_sequence = consensus_sequence?
erev 'The consensus sequence is: '
e
e " #{simp(the_consensus_sequence)}"
e
if the_consensus_sequence.include? '/'
try_to_find_and_report_a_simpler_consensus_sequence(
the_consensus_sequence
)
try_to_find_and_report_an_even_simpler_consensus_sequence(
the_consensus_sequence
)
if @be_verbose
erev 'Finally, we will display the above findings in a'
erev 'shorter variant:'
report_on_which_sequences_we_will_operate
display_an_even_simpler_consensus_sequence
e
end
end
end
end
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#reset ⇒ Object
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# File 'lib/bioroebe/utility_scripts/consensus_sequence.rb', line 60
def reset
super()
@array_consensus_positions = []
@be_verbose = true
@report_the_frequencies = true
end
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#return_A_or_C ⇒ Object
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# File 'lib/bioroebe/utility_scripts/consensus_sequence.rb', line 156
def return_A_or_C
%w( A C ).sample
end
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#return_A_or_T ⇒ Object
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# File 'lib/bioroebe/utility_scripts/consensus_sequence.rb', line 163
def return_A_or_T
%w( A T ).sample
end
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#return_A_or_T_or_C ⇒ Object
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# File 'lib/bioroebe/utility_scripts/consensus_sequence.rb', line 171
def return_A_or_T_or_C
%w( A T C ).sample
end
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#return_an_even_simpler_consensus_sequence(i = consensus_sequence?
) ⇒ Object
#
return_an_even_simpler_consensus_sequence
#
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# File 'lib/bioroebe/utility_scripts/consensus_sequence.rb', line 266
def return_an_even_simpler_consensus_sequence(
i = consensus_sequence?
)
_ = i.dup
if _.include? '[A/T/C]'
_.gsub!(/\[A\/T\/C\]/,
steelblue(return_A_or_T_or_C)+
remove_escape_sequence(orange)
)
end
if _.include? '[A/C]'
_.gsub!(/\[A\/C\]/,
steelblue(return_A_or_C)+
remove_escape_sequence(orange)
)
end
if _.include? '[A/T]'
_.gsub!(/\[A\/T\]/,
steelblue(return_A_or_T)+
remove_escape_sequence(orange)
)
end
_
end
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#run ⇒ Object
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# File 'lib/bioroebe/utility_scripts/consensus_sequence.rb', line 346
def run
report_on_which_sequences_we_will_operate
iterate_over_the_sequences
report_the_consensus_sequence
end
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# File 'lib/bioroebe/utility_scripts/consensus_sequence.rb', line 86
def set_input_sequences(i)
@input_sequences = [i].flatten
end
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#try_to_find_and_report_a_simpler_consensus_sequence(i = consensus_sequence?
) ⇒ Object
#
try_to_find_and_report_a_simpler_consensus_sequence
For a list of IUPAC codes there, you can use this link:
https://www.bioinformatics.org/sms/iupac.html
#
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# File 'lib/bioroebe/utility_scripts/consensus_sequence.rb', line 314
def try_to_find_and_report_a_simpler_consensus_sequence(
i = consensus_sequence?
)
erev "#{rev}We will try to find - and report - a simpler consensus sequence:"
i = orange(i)
i = i.dup if i.frozen?
if i.include? '[A/T/C]'
i.gsub!(/\[A\/T\/C\]/,
steelblue('H')+
remove_escape_sequence(orange)
)
end
if i.include? '[A/C]'
i.gsub!(/\[A\/C\]/,
steelblue('M')+
remove_escape_sequence(orange)
)
end
e
erev " #{i}"
e
end
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#try_to_find_and_report_an_even_simpler_consensus_sequence(i = consensus_sequence?
) ⇒ Object
#
try_to_find_and_report_an_even_simpler_consensus_sequence
#
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# File 'lib/bioroebe/utility_scripts/consensus_sequence.rb', line 294
def try_to_find_and_report_an_even_simpler_consensus_sequence(
i = consensus_sequence?
)
erev "#{rev}And the following is an even simpler consensus "\
"sequence (blue"
erev "denotes random, equal choice):"
_ = return_an_even_simpler_consensus_sequence(i)
e
erev " #{_}"
e
end
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