{:platform_error_rate=>0.02,
:primer_pairs=>
[{:region=>"RT",
:cdna=>
"GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTNNNNNNNNNNNCAGTCACTATAGGCTGTACTGTCCATTTATC",
:forward=>
"GCCTCCCTCGCGCCATCAGAGATGTGTATAAGAGACAGNNNNGGCCATTGACAGAAGAAAAAATAAAAGC",
:majority=>0,
:end_join=>true,
:end_join_option=>1,
:overlap=>0,
:TCS_QC=>true,
:ref_genome=>"HXB2",
:ref_start=>2648,
:ref_end=>3257,
:indel=>true,
:trim=>false},
{:region=>"PR",
:cdna=>
"GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTNNNNNNNNNCAGTTTAACTTTTGGGCCATCCATTCC",
:forward=>
"GCCTCCCTCGCGCCATCAGAGATGTGTATAAGAGACAGNNNNTCAGAGCAGACCAGAGCCAACAGCCCCA",
:majority=>0,
:end_join=>true,
:end_join_option=>3,
:TCS_QC=>true,
:ref_genome=>"HXB2",
:ref_start=>0,
:ref_end=>2591,
:indel=>true,
:trim=>true,
:trim_ref=>"HXB2",
:trim_ref_start=>2253,
:trim_ref_end=>2549},
{:region=>"IN",
:cdna=>
"GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTNNNNNNNNNNNATCGAATACTGCCATTTGTACTGC",
:forward=>"GCCTCCCTCGCGCCATCAGAGATGTGTATAAGAGACAGNNNNAAAAGGAGAAGCCATGCATG",
:majority=>0,
:end_join=>true,
:end_join_option=>2,
:overlap=>171,
:TCS_QC=>true,
:ref_genome=>"HXB2",
:ref_start=>4384,
:ref_end=>4751,
:indel=>false,
:trim=>false},
{:region=>"V1V3",
:cdna=>
"GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTNNNNNNNNNNNCAGTCCATTTTGCTYTAYTRABVTTACAATRTGC",
:forward=>
"GCCTCCCTCGCGCCATCAGAGATGTGTATAAGAGACAGNNNNTTATGGGATCAAAGCCTAAAGCCATGTGTA",
:majority=>0,
:end_join=>true,
:end_join_option=>1,
:overlap=>0,
:TCS_QC=>true,
:ref_genome=>"HXB2",
:ref_start=>6585,
:ref_end=>7205..7210,
:indel=>true,
:trim=>false},
{:region=>"P17",
:cdna=>
"GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTNNNNNNNNNNNCAGTCAACAAGGTTTCTGTCATCCAATTTTTTAC",
:forward=>
"GCCTCCCTCGCGCCATCAGAGATGTGTATAAGAGACAGNNNNGTCAGCCAAAATTACCCTATAGTGC",
:majority=>0,
:end_join=>true,
:end_join_option=>1,
:overlap=>0,
:TCS_QC=>true,
:ref_genome=>"HXB2",
:ref_start=>1196,
:ref_end=>1725,
:indel=>true,
:trim=>false}
]
}